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Biocare Medical polymer-based peroxidase staining method
Polymer Based Peroxidase Staining Method, supplied by Biocare Medical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peroxidase+based+method/polymer+based+peroxidase+staining+method/pm37549143-409-7-11
Average 90 stars, based on 1 article reviews
polymer-based peroxidase staining method - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

Staining:

Article Title: Vertical transmission of African-lineage Zika virus through the fetal membranes in a rhesus macaque ( Macaca mulatta ) model
Article Snippet: Sections were immunohistochemically stained using a proprietary polymer-based peroxidase staining method (Biocare Medical, Concord, CA, USA) and incubated with primary antibodies ( ) overnight at 4°C.

Article Title: Vertical transmission of African-lineage Zika virus through the fetal membranes in a rhesus macaque (Macaca mulatta) model.
Article Snippet: Sections were immunohistochemically stained using a proprietary polymer-based peroxidase staining method (Biocare Medical, Concord, CA, USA) and incubated with primary antibodies (S3 Table) overnight at 4 ̊C.

Article Title: Differential Localization of Flavin-Containing Monooxygenase (FMO) Isoforms 1, 3, and 4 in Rat Liver and Kidney and Evidence for Expression of FMO4 in Mouse, Rat, and Human Liver and Kidney Microsomes
Article Snippet: Sections were immunohistochemically stained using a proprietary polymer-based peroxidase staining method (Biocare Medical, Concord, CA).

Article Title: Utility of Ki-67, CD10, CD34, p53, CD117, and mast cell content in the differential diagnosis of cellular fibroadenomas and in the classification of phyllodes tumors of the breast.
Article Snippet: Adequate management of phyllodes tumors of the breast (PTB) remains a challenge because of the difficulty in correctly establishing preoperative diagnosis.. The aim of this study was to evaluate the usefulness of Ki-67, CD10, CD34, p53, CD117, and of the number of mast cells in the differential diagnosis of benign PTB and cellular fibroadenomas (CFs) as well as in the grading of PTB.. Fifty-one primary PTB and 14 CFs were examined by immunohistochemistry.When evaluating CD117 expression, higher epithelial expression was present in CF as well as an increased number of mast cells in benign PTB.

Article Title: Infection of the maternal-fetal interface and vertical transmission following low-dose inoculation of pregnant rhesus macaques (Macaca mulatta) with an African-lineage Zika virus.
Article Snippet: Sections were immunohistochemically stained using a proprietary polymer-based peroxidase staining method (Biocare Medical, Concord, CA): sections were blocked 20 min in Background Punisher (Biocare Medical) and incubated in 1:100 mouse anti-cytokeratin CAM5.2 monoclonal antibody (item 452M-94, Sigma-Aldrich, St. Louis, MO) or 1:1000 antiCD163 monoclonal antibody (Novus Biologicals, Centennial, CO. Ref NB110-40686) overnight at 4 degrees.

Article Title: Infection of the maternal-fetal interface and vertical transmission following low-dose inoculation of pregnant rhesus macaques ( Macaca mulatta ) with an African-lineage Zika virus
Article Snippet: CD56, CD68, and CD31 stained tissues sections were immunostained using the Benchmark XT autostainer (Ventana Medical Systems, Tucson, AZ, USA) using provided pre-diluted antibodies CD56, CD68, and CD31 (Ventana Medical Systems).

Polymer:

Article Title: Vertical transmission of African-lineage Zika virus through the fetal membranes in a rhesus macaque ( Macaca mulatta ) model
Article Snippet: Sections were immunohistochemically stained using a proprietary polymer-based peroxidase staining method (Biocare Medical, Concord, CA, USA) and incubated with primary antibodies ( ) overnight at 4°C.

Article Title: Vertical transmission of African-lineage Zika virus through the fetal membranes in a rhesus macaque (Macaca mulatta) model.
Article Snippet: Sections were immunohistochemically stained using a proprietary polymer-based peroxidase staining method (Biocare Medical, Concord, CA, USA) and incubated with primary antibodies (S3 Table) overnight at 4 ̊C.

Article Title: Differential Localization of Flavin-Containing Monooxygenase (FMO) Isoforms 1, 3, and 4 in Rat Liver and Kidney and Evidence for Expression of FMO4 in Mouse, Rat, and Human Liver and Kidney Microsomes
Article Snippet: Sections were immunohistochemically stained using a proprietary polymer-based peroxidase staining method (Biocare Medical, Concord, CA).

Article Title: Utility of Ki-67, CD10, CD34, p53, CD117, and mast cell content in the differential diagnosis of cellular fibroadenomas and in the classification of phyllodes tumors of the breast.
Article Snippet: Adequate management of phyllodes tumors of the breast (PTB) remains a challenge because of the difficulty in correctly establishing preoperative diagnosis.. The aim of this study was to evaluate the usefulness of Ki-67, CD10, CD34, p53, CD117, and of the number of mast cells in the differential diagnosis of benign PTB and cellular fibroadenomas (CFs) as well as in the grading of PTB.. Fifty-one primary PTB and 14 CFs were examined by immunohistochemistry.When evaluating CD117 expression, higher epithelial expression was present in CF as well as an increased number of mast cells in benign PTB.

Article Title: Infection of the maternal-fetal interface and vertical transmission following low-dose inoculation of pregnant rhesus macaques (Macaca mulatta) with an African-lineage Zika virus.
Article Snippet: Sections were immunohistochemically stained using a proprietary polymer-based peroxidase staining method (Biocare Medical, Concord, CA): sections were blocked 20 min in Background Punisher (Biocare Medical) and incubated in 1:100 mouse anti-cytokeratin CAM5.2 monoclonal antibody (item 452M-94, Sigma-Aldrich, St. Louis, MO) or 1:1000 antiCD163 monoclonal antibody (Novus Biologicals, Centennial, CO. Ref NB110-40686) overnight at 4 degrees.

Article Title: Infection of the maternal-fetal interface and vertical transmission following low-dose inoculation of pregnant rhesus macaques ( Macaca mulatta ) with an African-lineage Zika virus
Article Snippet: CD56, CD68, and CD31 stained tissues sections were immunostained using the Benchmark XT autostainer (Ventana Medical Systems, Tucson, AZ, USA) using provided pre-diluted antibodies CD56, CD68, and CD31 (Ventana Medical Systems).

Incubation:

Article Title: Vertical transmission of African-lineage Zika virus through the fetal membranes in a rhesus macaque ( Macaca mulatta ) model
Article Snippet: Sections were immunohistochemically stained using a proprietary polymer-based peroxidase staining method (Biocare Medical, Concord, CA, USA) and incubated with primary antibodies ( ) overnight at 4°C.

Article Title: Vertical transmission of African-lineage Zika virus through the fetal membranes in a rhesus macaque (Macaca mulatta) model.
Article Snippet: Sections were immunohistochemically stained using a proprietary polymer-based peroxidase staining method (Biocare Medical, Concord, CA, USA) and incubated with primary antibodies (S3 Table) overnight at 4 ̊C.

Article Title: Differential Localization of Flavin-Containing Monooxygenase (FMO) Isoforms 1, 3, and 4 in Rat Liver and Kidney and Evidence for Expression of FMO4 in Mouse, Rat, and Human Liver and Kidney Microsomes
Article Snippet: Sections were immunohistochemically stained using a proprietary polymer-based peroxidase staining method (Biocare Medical, Concord, CA).

Article Title: Utility of Ki-67, CD10, CD34, p53, CD117, and mast cell content in the differential diagnosis of cellular fibroadenomas and in the classification of phyllodes tumors of the breast.
Article Snippet: Adequate management of phyllodes tumors of the breast (PTB) remains a challenge because of the difficulty in correctly establishing preoperative diagnosis.. The aim of this study was to evaluate the usefulness of Ki-67, CD10, CD34, p53, CD117, and of the number of mast cells in the differential diagnosis of benign PTB and cellular fibroadenomas (CFs) as well as in the grading of PTB.. Fifty-one primary PTB and 14 CFs were examined by immunohistochemistry.When evaluating CD117 expression, higher epithelial expression was present in CF as well as an increased number of mast cells in benign PTB.

Article Title: Infection of the maternal-fetal interface and vertical transmission following low-dose inoculation of pregnant rhesus macaques (Macaca mulatta) with an African-lineage Zika virus.
Article Snippet: Sections were immunohistochemically stained using a proprietary polymer-based peroxidase staining method (Biocare Medical, Concord, CA): sections were blocked 20 min in Background Punisher (Biocare Medical) and incubated in 1:100 mouse anti-cytokeratin CAM5.2 monoclonal antibody (item 452M-94, Sigma-Aldrich, St. Louis, MO) or 1:1000 antiCD163 monoclonal antibody (Novus Biologicals, Centennial, CO. Ref NB110-40686) overnight at 4 degrees.

Article Title: Infection of the maternal-fetal interface and vertical transmission following low-dose inoculation of pregnant rhesus macaques ( Macaca mulatta ) with an African-lineage Zika virus
Article Snippet: CD56, CD68, and CD31 stained tissues sections were immunostained using the Benchmark XT autostainer (Ventana Medical Systems, Tucson, AZ, USA) using provided pre-diluted antibodies CD56, CD68, and CD31 (Ventana Medical Systems).



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Image Search Results


Expression of membrane RANKL on BC cell lines under the two different culture conditions. ( A ) Immunocytochemistry staining for mRANKL in the MDA-MB-231 cell line under both culture conditions (x 400 magnification). ( B ) Immunocytochemistry staining for mRANKL in the MCF-7 cell line under both culture conditions (x 400). Positive controls were performed in both BC cell lines incubated with anti-RANKL (cytoplasmatic RANKL = cRANKL, evaluated previously) and anti-AE1AE3 (a pan-cytokeratin epithelial marker). No staining was observed in both BC cell lines incubated with an irrelevant rabbit Ig Ab as a negative isotype control. Nuclei were counterstained with hematoxylin (purple).

Journal: Cancer Cell International

Article Title: Expression of osteoprotegerin, receptor activator of nuclear factor kappa-B ligand, tumor necrosis factor-related apoptosis-inducing ligand, stromal cell-derived factor-1 and their receptors in epithelial metastatic breast cancer cell lines

doi: 10.1186/1475-2867-12-29

Figure Lengend Snippet: Expression of membrane RANKL on BC cell lines under the two different culture conditions. ( A ) Immunocytochemistry staining for mRANKL in the MDA-MB-231 cell line under both culture conditions (x 400 magnification). ( B ) Immunocytochemistry staining for mRANKL in the MCF-7 cell line under both culture conditions (x 400). Positive controls were performed in both BC cell lines incubated with anti-RANKL (cytoplasmatic RANKL = cRANKL, evaluated previously) and anti-AE1AE3 (a pan-cytokeratin epithelial marker). No staining was observed in both BC cell lines incubated with an irrelevant rabbit Ig Ab as a negative isotype control. Nuclei were counterstained with hematoxylin (purple).

Article Snippet: According to the manufacturer’s recommendations, a peroxidase-based immunocytochemistry staining method (K0690, Dako) was used for primary Ab detection, and a 3,3′-diaminobenzidine tetrahydrochloride (DAB) substrate system (K3468, Dako) was used as the chromogen.

Techniques: Expressing, Immunocytochemistry, Staining, Incubation, Marker

Expression of membrane and cytoplasmic RANK (mRANK/cRANK), SDF-1 and CXCR4 in both BC cell lines. ( A ) Immunocytochemistry staining for RANK, SDF-1 and CXCR4 in MDA-MB-231 and MCF-7 cells grown under arrest and post-stimulation culture conditions (x 400 magnification). ( B ) The MDA-MB-231 and MCF-7 cell lines were incubated with irrelevant IgG1 and mouse Igs Abs as negative isotype controls (x 400 magnification). Nuclei were counterstained with hematoxylin (purple).

Journal: Cancer Cell International

Article Title: Expression of osteoprotegerin, receptor activator of nuclear factor kappa-B ligand, tumor necrosis factor-related apoptosis-inducing ligand, stromal cell-derived factor-1 and their receptors in epithelial metastatic breast cancer cell lines

doi: 10.1186/1475-2867-12-29

Figure Lengend Snippet: Expression of membrane and cytoplasmic RANK (mRANK/cRANK), SDF-1 and CXCR4 in both BC cell lines. ( A ) Immunocytochemistry staining for RANK, SDF-1 and CXCR4 in MDA-MB-231 and MCF-7 cells grown under arrest and post-stimulation culture conditions (x 400 magnification). ( B ) The MDA-MB-231 and MCF-7 cell lines were incubated with irrelevant IgG1 and mouse Igs Abs as negative isotype controls (x 400 magnification). Nuclei were counterstained with hematoxylin (purple).

Article Snippet: According to the manufacturer’s recommendations, a peroxidase-based immunocytochemistry staining method (K0690, Dako) was used for primary Ab detection, and a 3,3′-diaminobenzidine tetrahydrochloride (DAB) substrate system (K3468, Dako) was used as the chromogen.

Techniques: Expressing, Immunocytochemistry, Staining, Incubation

Expression of TRAIL, TRAIL-R1, TRAIL-R2, TRAIL-R3 and TRAIL-R4 in both BC cell lines. ( A ) Immunocytochemistry staining for TRAIL, TRAIL-R1, TRAIL-R2, TRAIL-R3 and TRAIL-R4 in the MDA-MB-231 and MCF-7 cell lines grown under arrest and post-stimulation culture conditions (x 400 magnification). ( B ) The MDA-MB-231 and MCF-7 cell lines were incubated with anti-AE1AE3 (positive control) and with irrelevant mouse Igs, goat IgG and IgG1 Abs as negative isotype controls (x 400 magnification). Nuclei were counterstained with hematoxylin (purple).

Journal: Cancer Cell International

Article Title: Expression of osteoprotegerin, receptor activator of nuclear factor kappa-B ligand, tumor necrosis factor-related apoptosis-inducing ligand, stromal cell-derived factor-1 and their receptors in epithelial metastatic breast cancer cell lines

doi: 10.1186/1475-2867-12-29

Figure Lengend Snippet: Expression of TRAIL, TRAIL-R1, TRAIL-R2, TRAIL-R3 and TRAIL-R4 in both BC cell lines. ( A ) Immunocytochemistry staining for TRAIL, TRAIL-R1, TRAIL-R2, TRAIL-R3 and TRAIL-R4 in the MDA-MB-231 and MCF-7 cell lines grown under arrest and post-stimulation culture conditions (x 400 magnification). ( B ) The MDA-MB-231 and MCF-7 cell lines were incubated with anti-AE1AE3 (positive control) and with irrelevant mouse Igs, goat IgG and IgG1 Abs as negative isotype controls (x 400 magnification). Nuclei were counterstained with hematoxylin (purple).

Article Snippet: According to the manufacturer’s recommendations, a peroxidase-based immunocytochemistry staining method (K0690, Dako) was used for primary Ab detection, and a 3,3′-diaminobenzidine tetrahydrochloride (DAB) substrate system (K3468, Dako) was used as the chromogen.

Techniques: Expressing, Immunocytochemistry, Staining, Incubation, Positive Control